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AB5589

Sigma-Aldrich

Anti-Potassium Channel Kv1.3 Antibody, extracellular domain

Chemicon®, from rabbit

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

rat, human

fabricante / nombre comercial

Chemicon®

técnicas

flow cytometry: suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... KCNA3(3738)

Especificidad

Recognizes Kv1.3, extracellular (Kcna3).

Inmunógeno

Epitope: extracellular domain
Highly purified peptide corresponding to residues 263-275 of human Kv1.3 (Accession P22001).

Aplicación

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Potassium Channel Kv1.3 Antibody, extracellular domain is validated for use in FC, WB for the detection of Potassium Channel Kv1.3.
Western blotting: 1:200-1:500 using ECL on rat brain membranes.

Indirect flow cytometry: 0.5-5 μg of antibody per 1x10e6cells.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Forma física

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Almacenamiento y estabilidad

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Nota de análisis

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Aquatic Chronic 3

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Luiz O Leiria et al.
The Journal of physiology, 591(9), 2259-2273 (2013-03-13)
We aimed to investigate the role of insulin in the bladder and its relevance for the development of overactive bladder (OAB) in insulin-resistant obese mice. Bladders from male individuals who were involved in multiple organ donations were used. C57BL6/J mice
Ameet A Chimote et al.
The Journal of biological chemistry, 287(3), 2055-2067 (2011-12-03)
Hypoxia in solid tumors contributes to decreased immunosurveillance via down-regulation of Kv1.3 channels in T lymphocytes and associated T cell function inhibition. However, the mechanisms responsible for Kv1.3 down-regulation are not understood. We hypothesized that chronic hypoxia reduces Kv1.3 surface
Zerrin Kuras et al.
PloS one, 7(8), e43859-e43859 (2012-09-07)
The migration of T lymphocytes is an essential part of the adaptive immune response as T cells circulate around the body to carry out immune surveillance. During the migration process T cells polarize, forming a leading edge at the cell

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