AB2047
Anti-Fibronectin Antibody
Chemicon®, from rabbit
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
Productos recomendados
origen biológico
rabbit
forma del anticuerpo
affinity purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
bovine
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
western blot: suitable
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Descripción general
Fibronectin (FN) is an extracellular adhesion molecule and it is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion.
Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey, http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}.
Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey, http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}.
Especificidad
Antibody reacts with bovine fibronectin, and demonstrates cross-reactivity of less than 0.1% with bovine collagens types I, III, IV by RIA at 1:10,000 dilution.
Inmunógeno
Fibronectin extracted and purified from bovine plasma.
Aplicación
Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA, IF, RIA, WB, IH(P).
Immunohistochemistry: 1:80 dilution for immunofluorescent staining of fresh frozen bovine skin and liver tissues. Acetone or methyl-carnoy fixation is also reactive.
Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 minutes), acetone or methyl-carnoy fixation; traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Immunoblotting: 1:1000 of 2% deoxycholate + 10% SDS, 6M urea extracted bovine cell cultures (Kinsella, 2000). Antibody demonstates the appropriate twin bands at ~220kDa.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 minutes), acetone or methyl-carnoy fixation; traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Immunoblotting: 1:1000 of 2% deoxycholate + 10% SDS, 6M urea extracted bovine cell cultures (Kinsella, 2000). Antibody demonstates the appropriate twin bands at ~220kDa.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Forma física
Format: Purified
Protein G affinity purified IgG fraction at 1.0 mg/mL in liquid PBS (0.01M phosphate, 0.09M NaCl) pH 7.2 with no preservatives.
Almacenamiento y estabilidad
Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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