AB1560
Anti-Opioid Receptor Antibody, δ, pain, NT
serum, Chemicon®
Sinónimos:
Anti-DOP, Anti-DOR, Anti-DOR1, Anti-OPRD
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
mouse, human, rat
fabricante / nombre comercial
Chemicon®
técnicas
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Especificidad
Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.
Inmunógeno
Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).
Epitope: N-terminus
Aplicación
Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.
Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neuroinflammation & Pain
Descripción de destino
36 and 72 kDa
Ligadura / enlace
Replaces: MAB5330
Forma física
UnPurified whole rabbit antiSerum containing no preservatives.
Unpurified
Almacenamiento y estabilidad
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análisis
Control
Spinal cord
Spinal cord
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Peripheral ?-opioid receptors attenuate the exercise pressor reflex.
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C M Cahill et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(19), 7598-7607 (2001-09-22)
Opioid receptors are known to undergo complex regulatory changes in response to ligand exposure. In the present study, we examined the effect of morphine on the in vitro and in vivo density and trafficking of delta opioid receptors (deltaORs). Prolonged
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