Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

70236-M

Sigma-Aldrich

BL21(DE3)pLysS Singles Competent Cells - Novagen

Escherichia coli, rod shaped

Sinónimos:

BL21(DE3) competent cells, Competent bacterial cells

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Seleccione un Tamaño

11 REACTIONS
214,00 €

214,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Todas las fotos(1)

Seleccione un Tamaño

Cambiar Vistas
11 REACTIONS
214,00 €

About This Item

Código UNSPSC:
41106202
NACRES:
NA.54

214,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

Nombre del producto

BL21(DE3)pLysS Singles Competent Cells - Novagen,

origen biológico

Escherichia coli

Nivel de calidad

100

fabricante / nombre comercial

Novagen®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

modo de crecimiento

adherent or suspension

morfología

rod shaped

técnicas

microbiological culture: suitable

transformación celular

transformation efficiency: >2×107 cfu/μg

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Componentes

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

Advertencia

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Preguntas

1–2 de 2 Preguntas  

  1. How many µL are in each tube of the singles?

    1 respuesta

    1. Each reaction tube contains 50 ul of competent cells. This product is available as a set of 11 or 22 tubes or reactions.

      ¿Le ha resultado útil?

  2. What is the difference between the strain expressing pLysE and pLysS?

    1 respuesta

    1. Products 69451-M and 70236-M are exactly the same competent cells. The only difference is in the packaging. The 70236 is already in 50uL aliquots, which are single-use, each aliquot is used for one reaction. This reference is available in two sizes of 11 or 22 reactions. While product 69451 is available in single 0.4 or 1mL volumes which may be thawed once and dispensed to smaller aliquots.
      The difference between the 69451-M/70236-M products and the CMC0015 BL21 is the type of plasmid that they express for the T7 lysozymes. The expression of T7 lysozyme reduces the basal expression of the target genes, inhibiting the T7 RNA polymerase. Specifically, with the expression of the plasmid pLysE (Ref. CMC0015) a higher expression of the levels of T7 Lysozime is obtained in comparison with the plasmid pLysS (ref. 69451-M/70236-M). Consequently, with reference CMC0015 there is even less risk of basal expression of the target protein in the absence of IPTG stimulation. This could be particularly relevant for toxic proteins that could affect competent cell viability.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico