616430

TGF-α, Human, Recombinant, E. coli

• Sinónimos: TGF-α, Human, Recombinant, E. coli, Transforming Growth Factor-α
• Número de CAS: 105186-99-0
• Número MDL: MFCD00082077
• Código UNSPSC: 12352202

Propiedades

• Ensayo: ≥97% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤100 pg/μg Endotoxin (pg/μg TGF-α)
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

Acciones bioquímicas o fisiológicas

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from 30% acetonitrile, 0.1% TFA.

Reconstitución

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

Otras notas

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany