574597-M

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

liquid, Calbiochem^®

• Sinónimos: Anti-SOD
• Código UNSPSC: 12352203

Propiedades

• origen biológico: sheep
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: liquid
• contiene: ≤0.1% sodium azide as preservative
• reactividad de especies: mouse, rat, human, monkey
• fabricante / nombre comercial: Calbiochem^®
• disponibilidad: not available in Canada,
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• isotipo: IgG
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.

Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.

Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O₂-) to molecular oxygen (O₂) and hydrogen peroxide (H₂O₂).

This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

Inmunógeno

Human

highly purified, human erythrocyte SOD Cu/Zn.

Aplicación

Immunoblotting (1:1000)

Immunoelectrophoresis (see comments)

Frozen sections (see comments)

Paraffin sections (see comments)

Free-floating sections (see comments)

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In glycine-buffered saline, pH 7.4.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Nota de análisis

Positive Control
Human red blood cell lysate

Otras notas

Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.

This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable