DMF: 0.5 mg/mL (Small yellow particles may be visible.) DMSO: 0.5 mg/mL
fluorescencia
λex ~378 nm λem ~503 nm
temp. de almacenamiento
2-8°C
Descripción general
A cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO•, ROO•, ONOO- and HO•. Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Dose-dependently images HOCl levels by two-photon fluorescence microscopy in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm).
A cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO·, ROO·, ONOO− and HO·). Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Also enables to monitor HOCl levels by two-photon image in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm) in a dose-dependent manner.
Acciones bioquímicas o fisiológicas
Cell permeable: yes
Primary Target hypochlorous acid
Envase
Packaged under inert gas
Advertencia
Toxicity: Standard Handling (A)
Reconstitución
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Otras notas
Xu, Q., et al. 2015. Angew. Chem. Int. Ed.54, 4890.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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