5.04744

Aphidicolin

≥98% (TLC), liquid, DNA polymerase α and δ inhibitor, Calbiochem

• Sinónimos: InSolution Aphidicolin
• Fórmula empírica (notación de Hill): C₂₀H₃₄O₄
• Número de CAS: 38966-21-1
• Peso molecular: 338.48
• Número MDL: MFCD00083214
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• product name: Aphidicolin, InSolution, ≥98%, 30 mM in DMSO, A cell-permeable tetracyclic diterpene antibiotic
• Nivel de calidad: 100
• Análisis: ≥98% (TLC)
• formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• temp. de almacenamiento: −70°C
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• Clave InChI: NOFOAYPPHIUXJR-APNQCZIXSA-N

Descripción

Descripción general

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific, non-competitive and reversible inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine (>Cat. No. 677181)-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
DNA polymerase α and δ

Reversible: yes

Envase

Packaged under inert gas

Advertencia

Toxicity: Irritant (B)

Forma física

A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin (Cat. No. 178273) in DMSO.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions.

Otras notas

Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene10, 185.
Urbani, L., et al. 1995. Exp. Cell Res.219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol.46, 1909.Hubermann, J.A. 1981. Cell23, 647.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 188.6 °F - (refers to pure substance)
• Punto de inflamabilidad (°C): 87 °C - (refers to pure substance)