437627

Lipopolysaccharide, E. coli O111: B4

from E. coli, , lyophilized, iNOS stimulator, Calbiochem^®

• Sinónimos: E. coli Lipopolysaccharide, E. coli O111:B4 LPS
• Código UNSPSC: 12352201
• NACRES: NA.77

Propiedades

• product name: Lipopolysaccharide, E. coli O111: B4, Lipopolysaccharide, E. coli O111: B4 stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes
• Nivel de calidad: 100
• formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤2.0% Protein; ≤2.5% nucleic acid
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Endotoxin lipopolysaccharides initiate a variety of biochemical cell activation pathways (PKC, cAMP-dependent protein kinase, arachidonate metabolism, protein myristoylation, and activation of G-proteins). These unique macromolecules have been extensively studied in order to elucidate and define relevant pathophysiological parameters of endotoxic shock, a profound life-threatening consequence of sepsis. More recently, these bacterial products have generated intense interest due to their pluropotential immunostimulatory activity as manifested by the activation of host cells (e.g. B lymphocytes, macrophages) to functional differentiation. Host cell activation by lipopolysaccharides produces a spectrum of hormone-active lymphokines and monokines, including interferons (α, β, and γ), interleukins (IL-1 and IL-6), tumor necrosis factor, platelet activating factor, and procoagulant tissue factor. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
Inducible nitric oxide synthase

Product does not compete with ATP.

Reversible: no

Advertencia

Toxicity: Harmful (C)

Nota de preparación

To achieve complete solubilization, heating solution to 37°C with intermittent vortexing or sonication may be necessary.

Reconstitución

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

Otras notas

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int. 35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol. 49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem. 60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.
Jackson, S.K., et al. 1992. Biochim. Biophys. Acta1135, 165.
O′Sullivan, M.G., et al. 1992. J. Biol. Chem.267, 14547.
Watanabe, K., et al. 1992. J. Cell. Physiol.150, 433.
Morrison, D.C., and Ryan, J.L. 1987. Annu. Rev. Med.38, 417.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H300
• Consejos de prudencia: P264 - P270 - P301 + P310 - P405 - P501
• Clasificaciones de peligro: Acute Tox. 2 Oral
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable