Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes Giα-1 and Giα-2 subunit proteins.
Recognizes Giα-1 and Giα-2 subunits. Does not cross-react with G3α-3 or Goα subunits.
Inmunógeno
a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian Giα-1 and Giα-2, conjugated to KLH
Aplicación
Immunoblotting (1:1000)
Advertencia
Toxicity: Standard Handling (A)
Forma física
In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Reconstitución
Following initial thaw, aliquot and freeze (-70°C).
Otras notas
Does not cross-react with Giα-3 and Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215. Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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