14-930-M

IRE1, active

• Código UNSPSC: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• mol peso: M_w 62 kDa
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable (kinase)
• Nº de acceso NCBI: NM_001433
• Nº de acceso UniProt: O75460

Descripción

Descripción general

Product Source: N-terminal 6His-tagged, recombinant, human IRE1 amino acids 465-end expressed by baculovirus in Sf21 insect cells.

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Envase

Also available in 250μg size as catalog # 14-930M and in bulk as catalog # 14-930-K.

Calidad

Routinely evaluated by phosphorylation of MBP.

Forma física

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Almacenamiento y estabilidad

6 months at -70°C

Otras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable