14-452-D

PDK1

• Código UNSPSC: 12352200
• eCl@ss: 32160405
• NACRES: NA.27

Propiedades

• descripción: protein target PDK1
• Nivel de calidad: 300
• Ensayo: ≥80%
• actividad específica: 5.02 pmol/min-μg
• concentración: 0.5 mg/mL
• técnicas: activity assay: suitable
• Nº de acceso UniProt: O15530
• Información sobre el gen: human ... PDPK1(5170)

Descripción

Descripción general

FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
SIZE: 556 amino acids; 63152 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.

Calidad

Evaluated by Kinase assay: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 EM ATP, 0.01% Brij-35 and 2 EM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.

Propiedades físicas

89 kDa

Forma física

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT

Almacenamiento y estabilidad

Stable for > 6 month at –80°C from date of receipt.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.