119107
ATP Assay Kit
Sinónimos:
Luciferin-Related Kit
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About This Item
Código UNSPSC:
41116133
Productos recomendados
uso
sufficient for 200 tests
Nivel de calidad
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
entrada
sample type soil
sample type blood
sample type urine
sample type milk
sample type sludge
método de detección
chemiluminescent
temp. de almacenamiento
−70°C
Descripción general
Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in the cell has been used as an indicator of cell death. In contrast, increased levels of ATP has been an indicator of cell proliferation. Calbiochem® brand ATP Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter.
The assay is based on the firefly luciferase-catalyzed oxidation of D-luciferin in the presence of ATP and oxygen, whereby the amount of ATP is quantified by the amount of light (hv) produced.
Componentes
One vial of Luciferase ATP Monitoring Enzyme, Enzyme Reconstitution Buffer, 1 bottle of Nucleotide Releasing Reagent, one vial of ATP Standard, and a user protocol.
Advertencia
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Especificaciones
Assay Time: 1 h
Principio
The assay can be fully automatic for high throughput (10 s/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.
Nota de preparación
Reagent Reconstitution1. Reconstitute ATP Monitoring Enzyme with 2ml of the Enzyme Reconstitution Buffer. Mix well by gentle pipetting. The reconstituted enzyme is stable for up to 2 months at 4°C.2. Prepare an ATP standard solution by dissolving the 1 mg ATP into 1 ml of H2O. The solution is stable for several weeks at -20°C.
Almacenamiento y estabilidad
Upon arrival store the entire contents of the kit at -70°C.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral
Órganos de actuación
Kidney
Código de clase de almacenamiento
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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