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Merck

MAK113

Sigma-Aldrich

ATPase/GTPase Activity Assay Kit

sufficient for 200 colorimetric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 200 colorimetric tests

método de detección

colorimetric

enfermedades relevantes

cancer

temp. de almacenamiento

2-8°C

Descripción general

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.

Principio

The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8B - Non-combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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