RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.
Aplicación
Glass TLC plates, RP-8 modified silica gel coated with flourescent indicator F254s. The RP-8 modified silica gel TLC plates come in a quantity of 25 size 5x10 cm for thin layer chromatography applications.
Ligadura / enlace
Replaces: 15684-1; 15684
Nota de análisis
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g d 50 (laser diffraction, size distribution): 9.5 - 11.5 µm Layer thickness: 200 - 270 µm Deviation of layer thickness per plate: ≤ 35 µm Chromatographic testings: A) Cholesterin test hRf-values - cholesterin, cholesterin test: 68 - 74 B) Steroid test - methyltestosterone, steroid test: 45 - 55 - Reichstein S, steroid test: 65 - 75 - hydrocortisone, steroid test: 75 - 91 Typical value determined on an activated plate (120°C, 15 min) Eluent a) aceton/ water (95/5 v/v) Eluent b) acetonitrile/ water (70/30 v/v)
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