1.06177

TBE buffer

10 x pH 8.3 tris-borate-EDTA buffer

• Sinónimos: 10X TBE running buffer, Tris-Borate EDTA buffer
• Código UNSPSC: 41105319
• NACRES: NA.25

Propiedades

• Formulario: liquid
• pH: 8.2-8.4 (20 °C in H₂O)
• densidad: 1.06 g/cm³ at 20 °C
• temp. de almacenamiento: 15-25°C

Descripción

Descripción general

Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO^- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH₂ and Tris-NH₃⁺). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg²⁺ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.

Nota de análisis

Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4

Información de seguridad

• Pictogramas: GHS07,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H360FD - H412
• Consejos de prudencia: P202 - P261 - P273 - P280 - P302 + P352 - P308 + P313
• Clasificaciones de peligro: Aquatic Chronic 3 - Repr. 1B - Skin Sens. 1
• Código de clase de almacenamiento: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable