06-1130-I

Anti-RNF168 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_689830
• Nº de acceso UniProt: Q8IYW5
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RNF168(165918)

Descripción

Descripción general

E3 ubiquitin-protein ligase RNF168 (UniProt Q8IYW5; also known as hRNF168, RING finger protein 168) is encoded by the RNF168 gene (Gene ID 165918) in human. Ubiquitylation/Ubiquitination is an essential early signal in the DNA damage repair process. Multiple E3 ligases, including RNF2 (RING2), RNF8 and RNF168, mediate the addition of K63-linked polyubiquitin chains to histone 2A (H2A) and H2AX, which in turn recruits DNA damage repair proteins, e.g. RAP80, BRCA1. In addition, SUMOylation and NEDDylation are also involved in the DNA damage response. RNF168 is an a dual specificity E3 ligase that mediates both the ubiquitination and NEDDylation of H2A and H2AX. The two types of modifications compete against each other and NEDD8 modification of H2A and H2AX blocks the recruitment of BRCA1 at DNA damage repair sites. RNF168 itself is also subjected to NEDD8 modification and NEDDylation of RNF168 is necessary for its ubiquitin ligase activity. Inhibition of RNF168 NEDDylation impairs its interaction with E2 enzyme Ubc13 (UBE2N). Likewise, downregulating RNF168 NEDDylation by the deNEDDylating enzyme NEDP1, or due to mutations of the NEDD8-conjugating enzyme UBC12, decreases H2A and H2AX ubiquitylation. RNF168 Mutations are linked to RIDDLE (radiosensitivity, immunodeficiency dysmorphic features and learning difficulties) syndrome.

Especificidad

This polyclonal antibody detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.

Inmunógeno

GST-tagged full-length human RNF168.

Aplicación

Anti-RNF168, Cat. No. 06-1130-I, is a highly specific rabbit polyclonal antibody that targets RNF168 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.

Immunoprecipitation Analysis: 20 µL from a representative lot immunoprecipitated RNF168 from 500 µg of human B-cell hybridoma SA13 cell lysate.

Calidad

Evaluated by Western Blotting in SA13 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RNF168 in 10 µg of human B-cell hybridoma SA13 cell lysate.

Descripción de destino

~75 kDa observed. 65.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Ligadura / enlace

Replaces: 06-1130

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable