05-23-3800

Endothelin 1, Human and Porcine

Endothelin 1, Human and Porcine, CAS 117399-94-7, is a 21-amino acid polypeptide with potent vasoconstrictive action. Activates phospholipase C in fibroblasts expressing ETA receptors.

• Sinónimos: Endothelin 1, Human and Porcine, ET-1, ET-1, CSCSSLMDKECVYFCHLDIIW, PPET1, Preproendothelin-1
• Fórmula empírica (notación de Hill): C₁₀₉H₁₅₉N₂₅O₃₂S₅
• Número de CAS: 117399-94-7
• Peso molecular: 2491.90
• Número MDL: MFCD00133305
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: Porcine; human
• Nivel de calidad: 100
• Ensayo: ≥96% (HPLC)
• Formulario: solid
• mol peso: 2491.9 g/mol
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: (Tightly closed Dry); OK to freeze; desiccated
• técnicas: cell culture | mammalian: suitable
• color: white to off-white
• solubilidad: 5% acetic acid: 0.5 mg/mL
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• InChI: 1S/C109H159N25O32S5/c1-12-56(9)87(107(163)125-76(109(165)166)39-60-43-113-65-24-18-17-23-63(60)65)134-108(164)88(57(10)13-2)133-99(155)75(42-85(143)144)123-94(150)70(36-54(5)6)118-97(153)73(40-61-44-112-52-114-61)121-103(159)80-49-169-168-48-64(111)89(145)126-77(45-135)102(158)131-81-50-170-171-51-82(105(161)132-86(55(7)8)106(162)124-72(38-59-26-28-62(138)29-27-59)95(151)120-71(96(152)130-80)37-58-21-15-14-16-22-58)129-91(147)67(30-31-83(139)140)116-90(146)66(25-19-20-33-110)115-98(154)74(41-84(141)142)122-92(148)68(32-34-167-11)117-93(149)69(35-53(3)4)119-100(156)78(46-136)127-101(157)79(47-137)128-104(81)160/h14-18,21-24,26-29,43-44,52-57,64,66-82,86-88,113,135-138H,12-13,19-20,25,30-42,45-51,110-111H2,1-11H3,(H,112,114)(H,115,154)(H,116,146)(H,117,149)(H,118,153)(H,119,156)(H,120,151)(H,121,159)(H,122,148)(H,123,150)(H,124,162)(H,125,163)(H,126,145)(H,127,157)(H,128,160)(H,129,147)(H,130,152)(H,131,158)(H,132,161)(H,133,155)(H,134,164)(H,139,140)(H,141,142)(H,143,144)(H,165,166)
• Clave InChI: ZUBDGKVDJUIMQQ-UHFFFAOYSA-N

Descripción

Descripción general

Research area: Cell Signaling

Endothelin 1 (ET-1) is a peptide hormone[1] located in the endothelial cells[2] and belongs to the endothelin family.[1] It is a derivative of pre-pro-ET-1 which is later converted to mature ET-1 by a group of ET-converting enzymes (ECEs), endopeptidases, chymases, and non-ECE metalloproteinases.[2] The EDN1 gene is mapped to the human chromosome at 6p24.1.[3]

Aplicación

Endothelin 1, Human and Porcine - CAS 117399-94-7 – Calbiochem has been used:

• to study its effects on the production of erythropoietin under normoxia and carbon monoxide-induced hypoxia in rat models[4]
• as a protein kinase C (PKC) agonist to study its effects on the phosphorylation of myofilament proteins in rat myocytes[5]
• to induce a hypertrophic-like state to study its effects on contraction pressure in normal and mutated human induced pluripotent stem cells (hiPSC)-derived cardiomyocytes[6]

Acciones bioquímicas o fisiológicas

Endothelin 1 (ET-1) acts as a mediator of paracrine and autocrine functions. The production of ET-1 is upregulated under pathophysiological conditions and is produced in several types of cells such as cardiac myocytes, vascular smooth muscle cells, and inflammatory cells like leukocytes and macrophages. ET-1 is a potent vasoconstrictor peptide that is stimulated by risk factors in several types of cells for cardiovascular diseases.[2] It is involved in many biological effects such as pro-inflammatory actions, platelet activation, stimulation of free radicals, and mitogenic and proliferative effects.[2] ET-1 plays a role in pulmonary physiology, neurological function, autoimmune disorders, transportation of electrolytes and fluids, and cancer. It is an essential component for the development of embryonic neural crest-derived tissues.[1] ET-1 is involved in osteogenesis by promoting osteoblast proliferation by activating Wnt signaling pathway.[7]

Envase

Yes

Advertencia

Toxicity: Standard Handling (A)

Secuencia

H-Cys¹-Ser-Cys³-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys¹¹-Val-Tyr-Phe-Cys¹⁵-His-Leu-Asp-Ile-Ile-Trp-OH (disulfide bonds: 1 → 15; 3 → 11)

Forma física

Supplied as an acetate salt.

Nota de preparación

Dissolve peptide initially at 8 mg/ml in 80% cold acetic acid, then dilute with distilled water to achieve 5% acetic acid and 0.5 mg/ml peptide.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.

Otras notas

Cesari, M., et al. 1996. Am. Heart J.132, 1236.
Gresser, O., et al 1996. Biochem. Biophys. Res. Commun.224, 169.
Murata, S., et al. 1995. J. Pharmacol. Exp. Ther. 274, 1524.
Hexum, T.D., et al. 1990. Biochem. Biophys. Res. Commun. 167, 294.
Itoh, Y., et al. 1988. FEBS Lett. 231, 440.
Yanagisawa, M., et al. 1988. Nature 332, 411.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable