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05-1085

Sigma-Aldrich

Anti-IRS1 Antibody, clone 4.2.2

clone 4.2.2, from mouse

Sinónimos:

insulin receptor substrate 1

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100 μG
554,00 €

554,00 €

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100 μG
554,00 €

About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

554,00 €

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origen biológico

mouse

Nivel de calidad

100

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

4.2.2, monoclonal

reactividad de especies

pig, canine, human, mouse, rat, monkey, bovine

técnicas

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG2aκ

Nº de acceso NCBI

NP_005535.1

Nº de acceso UniProt

P35568

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

bovine ... Irs1(538598)
dog ... Irs1(486148)
human ... IRS1(3667)
mouse ... Irs1(16367)
pig ... Irs1(100512686)
rat ... Irs1(25467)
rhesus monkey ... Irs1(707870)

Categorías relacionadas

Descripción general

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

Especificidad

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
This antibody recognizes IRS1.

Inmunógeno

Synthetic peptide corresponding to amino acids 431-446 of mouse IRS1.

Aplicación

This Anti-IRS1 Antibody, clone 4.2.2 is validated for use in WB, IP, IC for the detection of IRS1.

Calidad

Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

Descripción de destino

~185 kDa

Forma física

Format: Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4),150 mM NaCl with 0.05% sodium azide.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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