04-958

Anti-Beta Catenin Antibody, clone 7F7.2

clone 7F7.2, Upstate^®, from mouse

• Sinónimos: Beta Catenin, Catenin (cadherin-associated protein), beta 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 7F7.2, monoclonal
• reactividad de especies: rat, mouse, human
• fabricante / nombre comercial: Upstate^®
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001895
• Nº de acceso UniProt: P35222
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Descripción

Descripción general

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy, HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Especificidad

This antibody recognizes beta catenin.

Inmunógeno

Full length, GST-tagged recombinant beta catenin.

Aplicación

Western Blot Analysis: 0.5-2 µg/mL of this lot detected beta catenin in HEK293 cell lysate.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Beta Catenin Antibody, clone 7F7.2 is validated for use in WB, IC for the detection of Beta Catenin.

Calidad

Routinely evaluated by western blot on HEK293 cell lysate.

Descripción de destino

92 kDa

Ligadura / enlace

Replaces: 04-1002

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HEK293 cell lysate, PC12 cell lysate, NIH/3T3 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable