04-1467

Anti-PABP 1 Antibody, clone 10E10

clone 10E10, from mouse

• Sinónimos: PABP 1, Poly(A)-binding protein 1, poly(A) binding protein, cytoplasmic 1, poly(A) binding protein, cytoplasmic 2, poly(A)-binding protein, cytoplasmic 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 10E10, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_002559.2
• Nº de acceso UniProt: P11940
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PABPC1(26986)

Descripción

Descripción general

Poly(A)-binding protein (PABP 1) is an abundant protein that interacts with the 3′ poly(A) tail of most eukaryotic mRNAs. In vivo this protein is involved in many aspects of mRNA metabolism, including mRNA stability, translationally coupled mRNA turnover, protein biosynthesis, and ribosome biogenesis. PABP1 shuttles between the cytoplasm and the nucleus and may bind to cytoplasmic RNA sequences other than poly(A). PABP1 accumulates in the nucleus if transcription is inhibited, suggesting that nuclear export is coupled to active transcription.

Especificidad

This antibody recognizes PABP 1.

Inmunógeno

Epitope: Unknown

Recombinant protein corresponding to human PABP 1.

Aplicación

Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & IP.

Immunoprecipitation:
Cited in Thoma, C., et al. (2008) RNA. 14:1579-1589, and Buchet-Poyau, K., et al.(2007) Nuc. Acids Res. 35:1289-1300 using a representative lot.

Immunocytochemistry:
Cited in Afonina, E, et al. (1998) J. Biol. Chem., 273: 13015-21 (1998) using a representative lot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Calidad

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected PABP 1 in 10 µg of HeLa cell lysate.

Descripción de destino

~ 71 kDa

Ligadura / enlace

Replaces: 05-847

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable