04-080

Anti-BAF250a/ARID1a Antibody, clone PSG3

Name: Anti-BAF250a/ARID1a Antibody, clone PSG3
Brand: MM

clone PSG3, Upstate^®, from mouse

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UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

PSG3, monoclonal

Application:

IF, WB

Citations:

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PSG3, monoclonal

species reactivity

human

manufacturer/tradename

Upstate^®

technique(s)

immunofluorescence: suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_006015.4, NM_139135.2

UniProt accession no.

O14497

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ARID1A (8289)

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Este artículo	05-647	SAB2108761	HPA005456
Sigma-Aldrich 04-080 Anti-BAF250a/ARID1a Antibody, clone PSG3	Sigma-Aldrich 05-647 Anti-Tyrosinase Antibody, clone T311	Sigma-Aldrich SAB2108761 Anti-SLC25A4	Sigma-Aldrich HPA005456 Anti-ARID1A antibody produced in rabbit
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
species reactivity human	species reactivity human	species reactivity dog, guinea pig, bovine, mouse, human, rabbit, rat, sheep, goat	species reactivity human
antibody form purified immunoglobulin	antibody form purified antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone PSG3, monoclonal	clone T311, monoclonal	clone polyclonal	clone polyclonal
Gene Information human ... ARID1A (8289)	Gene Information -	Gene Information human ... SLC25A4(291)	Gene Information human ... ARID1A(8289)
technique(s) immunofluorescence: suitable, western blot: suitable	technique(s) immunohistochemistry: suitable, western blot: suitable	technique(s) immunoblotting: suitable, immunohistochemistry: suitable	technique(s) immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500, western blot: 0.04-0.4 μg/mL

General description

~270 kDa

Immunogen

GST fusion protein corresponding to 419 residues in the mid-portion of human BAF250a/ARID1a

Application

Anti-BAF250a/ARID1a Antibody, clone PSG3 is an antibody against BAF250a/ARID1a for use in WB, ChIP, IC & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Transcription Factors

Biochem/physiol Actions

BAF250a/ARID1a

Physical form

100 μg of Protein A purified mouse monoclonal IgG in 100μl of 1X PBS, pH 7.0, 0.1% Azide.

Format: Purified

Protein A purified

Preparation Note

2 years at -20°C from date of shipment

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Referencia del producto

Descripción

Precios

ZRB1280

Anti-ARID1A Antibody, clone 1C3 ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Clase de almacenamiento

12 - Non Combustible Liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.

Chandler, RL; Damrauer, JS; Raab, JR; Schisler, JC; Wilkerson, MD; Didion, JP; Starmer et al.

Nature Communications null

Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors.

Hiroko Inoue et al.

The Journal of biological chemistry, 277(44), 41674-41685 (2002-08-30)

The mammalian SWI/SNF-related complexes facilitate gene transcription by remodeling chromatin using the energy of ATP hydrolysis. The recruitment of these complexes to promoters remains poorly understood and may involve histone modifications or direct interactions with site-specific transcription factors or other

Novel SWI/SNF chromatin-remodeling complexes contain a mixed-lineage leukemia chromosomal translocation partner.

Zuqin Nie et al.

Molecular and cellular biology, 23(8), 2942-2952 (2003-04-01)

The SWI/SNF family of chromatin-remodeling complexes has been discovered in many species and has been shown to regulate gene expression by assisting transcriptional machinery to gain access to their sites in chromatin. Several complexes of this family have been reported

Essential role of ARID2 protein-containing SWI/SNF complex in tissue-specific gene expression.

Xu, F; Flowers, S; Moran, E

The Journal of Biological Chemistry null

ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice.

Radhika Mathur et al.

Nature genetics, 49(2), 296-302 (2016-12-13)

Genes encoding subunits of SWI/SNF (BAF) chromatin-remodeling complexes are collectively mutated in ∼20% of all human cancers. Although ARID1A is the most frequent target of mutations, the mechanism by which its inactivation promotes tumorigenesis is unclear. Here we demonstrate that

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04-080	04053252669422

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