199303

Resazurin sodium salt

certified by the Biological Stain Commission

• Sinónimos: 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
• Fórmula empírica (notación de Hill): C₁₂H₆NNaO₄
• Número de CAS: 62758-13-8
• Peso molecular: 251.17
• Beilstein: 3794971
• Número CE: 263-718-5
• Número MDL: MFCD00005036
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24851905
• NACRES: NA.47

Propiedades

• grado: certified by the Biological Stain Commission
• Nivel de calidad: 200
• formulario: powder
• composición: Dye content, 75%
• solubilidad: 1 M NH4OH: 1 mg/mL
• λmáx.: 380 nm (2nd); 598 nm
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
• InChI: 1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
• Clave InChI: IVGPGQSSDLDOLH-UHFFFAOYSA-M

Descripción

Descripción general

Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.

Aplicación

Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:

• the measurement of metabolic activity and the proliferation of living cells
• the detection of bacterial reduction in milk
• in viability assays as a component of culture media for the detection of viable bacteria
• to determine the number of motile sperm in sheep semen
• to assay lactate dehydrogenase
• as an analytical reagent to quantify L-glutamate

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315,H319,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves