D219

Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution

• Synonym(s): Anti-CACNA2, Anti-CACNL2A, Anti-CCHL2A, Anti-LINC01112, Anti-lncRNA-N3
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01323189
• Conjugate: unconjugated
• Clone: 20A, monoclonal
• Application: IHC (f), WB
• Citations: 25

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 20A, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 143 kDa (reduced), antigen 220 kDa (non-reduced)
• species reactivity: rat, mouse, rabbit, guinea pig
• technique(s): immunohistochemistry (frozen sections): 1:500, western blot: 1:500
• isotype: IgG2a
• UniProt accession no.: O08532
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Cacna2d1(12293) ; rat ... Cacna2d1(25399)

Description

General description

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

Immunogen

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:

• immunofluorescent histochemistry
• surface plasmon resonance binding
• immunohistochemistry (IHC)
• immunoblotting

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

Biochem/physiol Actions

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

Reacts specifically with the α₂ subunit of the DHP receptor. Strong reactivity is observed with the α₂ subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Other Notes

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)