C4733
BID, Caspase-8-cleaved from mouse
≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 μg | Please contact Customer Service for Availability | €838.00 |
About This Item
€838.00
Please contact Customer Service for Availability
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Bid(12122)
General description
Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.
Biochem/physiol Actions
Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.
Physical form
0.2 μm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.
Analysis Note
Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.
1 of 1
This Item | |||
|---|---|---|---|
| recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli (C-terminal histidine tagged) | recombinant expressed in E. coli (C-terminal histidine-tagged) |
| assay ≥95% (SDS-PAGE) | assay - | assay ≥90% (SDS-PAGE) | assay ≥85% (SDS-PAGE) |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form buffered aqueous solution | form - | form buffered aqueous solution | form buffered aqueous glycerol solution |
| storage temp. −20°C | storage temp. - | storage temp. −70°C | storage temp. −70°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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J Zha et al.
Science (New York, N.Y.), 290(5497), 1761-1765 (2000-12-02)
Many apoptotic molecules relocate subcellularly in cells undergoing apoptosis. The pro-apoptotic protein BID underwent posttranslational (rather than classic cotranslational) N-myristoylation when cleavage by caspase 8 caused exposure of a glycine residue. N-myristoylation enabled the targeting of a complex of p7
X Luo et al.
Cell, 94(4), 481-490 (1998-09-04)
We report here the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as Fas and TNF. Peptide mass fingerprinting identified this protein
M C Wei et al.
Genes & development, 14(16), 2060-2071 (2000-08-19)
TNFR1/Fas engagement results in the cleavage of cytosolic BID to truncated tBID, which translocates to mitochondria. Immunodepletion and gene disruption indicate BID is required for cytochrome c release. Surprisingly, the three-dimensional structure of this BH3 domain-only molecule revealed two hydrophobic
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C4733-50UG | 04061838070470 |