C0888

Clostripain from Clostridium histolyticum

≥20 units/mg solid

• Synonym(s): Clostridiopeptidase B, Proteinase from Clostridium histolyticum
• CAS Number: 9028-00-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-822-2
• MDL number: MFCD00130814
• EC Number: 3.4.22.8 (BRENDA, IUBMB)
• Specific activity: ≥20 units/mg solid

Properties

• form: lyophilized powder
• Quality Segment: 100
• specific activity: ≥20 units/mg solid
• mol wt: 15.4 kDa, 41.7 kDa
• impurities: salt, essentially free
• storage temp.: 2-8°C

Description

Application

Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).[1]

Biochem/physiol Actions

Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.[2]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.

Purified, essentially salt-free

Other Notes

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)