The concentration of this product varies from lot to lot and may range from 2.0 - 5.0 mg/mL. This lot-specific concentration if reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/b8895#product-documentation
B8895
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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0.5 ML
€110.00
1 ML
€94.50
2 ML
€457.80
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0.5 ML
€110.00
1 ML
€94.50
2 ML
€457.80
About This Item
biological source
goat
conjugate
biotin conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800
western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
storage temp.
−20°C
target post-translational modification
unmodified
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General description
IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Specificity
Binds all rabbit Igs.
Immunogen
Purified rabbit IgG
Application
Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in
- Immunofluorescence[1]
- Immunohistochemistry
- Western blot
- Direct enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.
Other Notes
Antibody adsorbed with human IgG.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Preparation Note
Adsorbed to reduce background staining with human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Jeffery M Vahrenkamp et al.
Cell reports, 22(11), 2995-3005 (2018-03-15)
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Feng Yang et al.
Cancer research, 65(19), 8887-8895 (2005-10-06)
Our previous studies have defined reactive stroma in human prostate cancer and have developed the differential reactive stroma (DRS) xenograft model to evaluate mechanisms of how reactive stroma promotes carcinoma tumorigenesis. Analysis of several normal human prostate stromal cell lines
Ting Jiang et al.
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<BIG>Lei L, et al.</BIG>
Sensors and Actuators B, Chemical, 273, 35-40 (2018)
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