59983

Josamycin

≥90% (HPLC)

• Synonym(s): Leucomycin A3
• Empirical Formula (Hill Notation): C₄₂H₆₉NO₁₅
• CAS Number: 16846-24-5
• Molecular Weight: 827.99
• NACRES: NA.85
• PubChem Substance ID: 57651709
• UNSPSC Code: 51102829
• EC Number: 240-871-6
• MDL number: MFCD00211043

Properties

• Quality Level: 200
• assay: ≥90% (HPLC)
• form: powder
• color: white to slightly yellow
• antibiotic activity spectrum: Gram-positive bacteria
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: CO[C@H]1[C@@H](CC(=O)O[C@H](C)C\C=C\C=C\[C@H](O)[C@H](C)C[C@H](CC=O)[C@@H]1O[C@@H]2O[C@H](C)[C@@H](O[C@H]3C[C@@](C)(O)[C@@H](OC(=O)CC(C)C)[C@H](C)O3)[C@@H]([C@H]2O)N(C)C)OC(C)=O
• InChI: 1S/C42H69NO15/c1-23(2)19-32(47)56-40-27(6)53-34(22-42(40,8)50)57-37-26(5)54-41(36(49)35(37)43(9)10)58-38-29(17-18-44)20-24(3)30(46)16-14-12-13-15-25(4)52-33(48)21-31(39(38)51-11)55-28(7)45/h12-14,16,18,23-27,29-31,34-41,46,49-50H,15,17,19-22H2,1-11H3/b13-12+,16-14+/t24-,25-,26-,27+,29+,30+,31-,34+,35-,36-,37-,38+,39+,40+,41+,42-/m1/s1
• InChI key: XJSFLOJWULLJQS-NGVXBBESSA-N

Description

General description

Chemical structure: macrolide

Application

Josamycin is used to study the modification of phagocytosis and cytokine production by macrolide antibiotics[1], immunomodulatory effects, the suppression of matrix metalloproteinase production[2] as well as study bacteria protein synthesis at the level of the the 23S rRNA, 50S ribosomal subunit.[3]

Biochem/physiol Actions

Josamycin inhibits bacterial protein biosynthesis by inhibiting peptidyltransferase and ribosomal translocation, and depleting the intracellular pools of aminoacyl-tRNAs available for protein synthesis by drop-off and incomplete peptidyl-tRNA hydrolase activity.[4] It slows down formation of the first peptide bond of a nascent peptide in an amino acid-dependent way and inhibits formation of the second or third peptide bond.[5]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)