45245
Eosin 5-isothiocyanate
≥95.0% (UV)
Synonym(s):
EITC
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About This Item
Empirical Formula (Hill Notation):
C21H7Br4NO5S
CAS Number:
Molecular Weight:
704.96
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:
assay
≥95.0% (UV)
fluorescence
λex 521; λem 544 (pH 9.0)
storage temp.
2-8°C
SMILES string
OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S
InChI
1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)
InChI key
MCXYFYWNOWPDMA-UHFFFAOYSA-N
Application
Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.
signalword
Danger
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
flash_point_f
Not applicable
hcodes
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Articles
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
J W Erickson et al.
Biochemistry, 34(27), 8693-8700 (1995-07-11)
In this work, we have used resonance energy transfer to determine the relative positions of a reactive cysteine residue on the gamma subunit of the retinal cyclic GMP phosphodiesterase (gamma PDE) and a reactive lysine residue on the alpha subunit
Photohemolysis of human erythrocytes labeled in band 3 with eosin-isothiocyanate.
J P Pooler et al.
Photochemistry and photobiology, 44(4), 495-499 (1986-10-01)
A D Horowitz et al.
Biochemistry, 32(37), 9513-9523 (1993-09-21)
The self-association of pulmonary surfactant protein SP-C in lipid vesicles was studied using fluorescence energy transfer. Bovine SP-C was labeled with two fluorescent probes, succinimidyl 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoate and eosin isothiocyanate, on the amino terminus of the protein, producing NBD-SP-C and EITC-SP-C