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Key Documents

SAB3500356

Sigma-Aldrich

Anti-MDA5 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-IFIH1, Anti-Interferon-induced with helicase C domain protein 1, Anti-Melanoma differentiation-associated protein 5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

predicted mol wt 113 kDa

species reactivity

mouse, human

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IFIH1(64135)

General description

Melanoma differentiation-associated protein 5 (MDA5), also known as interferon induced with helicase C domain 1 (IFIH1), is a cytosolic RNA sensor. It is encoded by the gene mapped to human chromosome 2q24.2. The encoded protein is a member of the retinoic acid-inducible gene I-like receptor family. MDA5 is characterized with both caspase recruitment domain and putative DExH group RNA helicase domains.

Immunogen

MDA5 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human MDA5.

Application

Anti-MDA5 antibody produced in rabbit has been used in western blot analysis.

Biochem/physiol Actions

Melanoma differentiation-associated protein 5 (MDA5) plays a vital role in antiviral innate immune responses. In addition, the encoded protein also activates interferon regulatory factors 3 and 7 and nuclear factor-κB (NF-κB) via a mitochondrial viral signaling protein, inducing antiviral and proapoptotic responses, which is associated with various autoimmune diseases, such as type 1 diabetes (T1D), Graves′ disease (GD), multiple sclerosis (MS), psoriasis and rheumatoid arthritis (RA). Polymorphism in the gene is associated with the development of generalized vitiligo (GV).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Linkage

The action of this antibody can be blocked using blocking peptide SBP3500356.

Physical form

Supplied in PBS with 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mitophagy Enhances Oncolytic Measles Virus Replication by Mitigating DDX58/RIG-I-Like Receptor Signaling
Xia M, et al.
Journal of Virology, 88(9), 5152-5164 (2014)
Mao Xia et al.
Journal of virology, 88(9), 5152-5164 (2014-02-28)
The success of future clinical trials with oncolytic viruses depends on the identification and the control of mechanisms that modulate their therapeutic efficacy. In particular, little is known about the role of autophagy in infection by attenuated measles virus of
mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties
Kang D C, et al.
Proceedings of the National Academy of Sciences of the USA, 99(2), 637-642 (2002)
Association Analyses Identify Three Susceptibility Loci for Vitiligo in the Chinese Han Population
Tang X F, et al.
The Journal of Investigative Dermatology, 133(2), 403-410 (2013)
Genetic susceptibility to vitiligo: Recent progress from genome-wide association studies
Zhang Z, et al.
Dermatologica Sinica, 32(4), 225-232 (2014)

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