HPLC Analysis of Glycan and Isomers on Proteomix^® SAX-NP5, High Resolution Separation

column

Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)

mobile phase

(A) 2.5% (v/v) acetic acid, 0.5% TEA in water; 0.5% acetic acid in acetonitrile

gradient

0-100% B in 60 min

flow rate

0.3 mL/min

detector

Fluorescence, Ex/Em=360/425 nm

sample

2-AA (anthranilic acid) labeled N-linked oligosaccharide profiling of an IgG1 sample