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ZMS1221

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Anti-LAMP2 Antibody, clone H4B4 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

CD107 antigen-like family member B, CD107b, LAMP-2, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

H4B4, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 44.9 kDa
observed mol wt ~105 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 25

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

Storage temp.

2-8°C

Gene Information

human ... LAMP2(3920)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone H4B4 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects LAMP-2.

Immunogen

Human adherent peripheral blood cells.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected LAMP2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected LAMP2 in lysate from U937 cells and 1:1,000 dilution in lysate from Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LAMP2 in Human liver tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected LAMP2 in one million U937 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lysosome-associated membrane glycoprotein 2 (UniProt: P13473; also known as LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, CD107b) is encoded by the LAMP2 gene (Gene ID: 3920) in human. LAMP-2 is a single-pass type I, heavily glycosylated, membrane protein found in plasma membrane, endosomes, and lysosomes. It is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains a lumenal domain (aa 29-375), a transmembrane domain (aa 376-399), and a short cytoplasmic tail (aa 400-410). It is an important component of lysosomal membranes and is involved in both autophagy and lysosomal membrane permeabilization. LAMP-2 plays an important role in chaperone-mediated autophagy. It is required for the fusion of autophagosomes with lysosomes during autophagy. It also plays a role in lysosomal protein degradation in response to starvation. It functions by binding target proteins, such as GAPDH and MLLT11, and targets them for lysosomal degradation. In cancer cells, chronic acidosis-induced upregulation of LAMP-2 has been reported that protects these cells from acid-induced hydrolysis and promotes their survival via chaperone-mediated autophagy. Loss of LAMP-2 is reported to aggravate oxidative stress through the obstruction of reactive oxygen species clearance. Three different isoforms of LAMP-2 have been described that are produced by alternative splicing. Mutations in LAMP2 gene have been linked to Danon disease that is characterized by cardiomyopathy, vascular myopathy, and mental retardation. It is often associated with accumulation of glycogen in muscle and lysosomes. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cui, L., et al. (2020). Front. Cell Dev. Biol. 8; 31; Qin, X., et al. (2017). Biochem. Biophys. Res. Commun. 488(1); 177-181; Cuervo, AM., and Dice, JF. (2000). J. Cell Sci. 113(24); 4441-4450).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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