In undiluted form or diluted to working concentration, this material may be stored at -20°C in aliquots up to the recommended retest date for the specific lot. This data is published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles. The solution may be transferred to smaller vials in working volumes for long-term storage. Plastic vials are recommended as this protein tends to adhere to glass. Please see the link below to access the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/409/686/l2020pis-ms.pdf
L2020
Mouse Laminin
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
1 of 4 reviewers received a sample product or took part in a promotion
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 0.2 μm filtered, liquid, 1-2 mg/mL, suitable for cell culture
Sinónimos:
Laminina
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About This Item
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Nombre del producto
Laminina from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
origen biológico
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Nivel de calidad
Línea del producto
BioReagent
Formulario
aqueous solution
mol peso
A subunit 400 kDa
B1 subunit 210 kDa
B2 subunit 200 kDa
envase
pkg of 1 mg
concentración
1-2 mg/mL in Tris-buffered saline
técnicas
cell culture | mammalian: suitable
cobertura de la superficie
1‑2 μg/cm2
impurezas
Microbial Contamination, passes test
Nº de acceso NCBI
Especificidad de unión
Peptide Source: Collagen
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Información sobre el gen
mouse ... Lama1(16772) , Lamb1(16777) , Lamc1(226519) , Lamc2(16782)
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Descripción general
Las lamininas son glucoproteínas adhesivas no colagenosas[1] con un peso molecular sustancial. Son proteínas heterotrímeras con tres polipéptidos unidos por puentes de disulfuro conocidos como cadenas α, β y γ.[2] Los múltiples dominios polipeptídicos de las lamininas pueden unirse a varios receptores de la superficie celular, como diversos dímeros de integrina, distroglucano y sindecanos, y a otros componentes de la matriz extracelular (ECM). Esta glucoproteína trímera multidominio de la matriz extracelular es el principal componente de la lámina basal que propicia la adhesión, la proliferación y la diferenciación.
Aplicación
La laminina de la membrana basal del sarcoma murino Engelbreth-Holm-Swarm se ha utilizado en:
La laminina soporta el crecimiento y la diferenciación de muchos tipos de células, como las células epiteliales, endoteliales, neuronales, musculares y hepáticas. Se recomienda su uso como substrato de cultivo celular a 1-2 μg/cm2. La concentración óptima depende del tipo de célula, así como de la aplicación o de los objetivos experimentales.
Acciones bioquímicas o fisiológicas
Las proteínas de la laminina son componentes integrales del andamiaje estructural de los tejidos animales. Se asocian con el colágeno de tipo IV a través de entactina y perlecán y se unen a las membranas celulares a través de receptores de integrina, complejos de glucoproteínas y distroglucanos y glucoproteínas del grupo sanguíneo luterano. La laminina tiene dominios activos para la unión del colágeno, la adhesión celular, la unión de la heparina y el fragmento de crecimiento de las neuronas.
Componentes
La laminina es una glucoproteína trimérica multidominio de matriz extracelular y es el principal componente no colágeno de la lámina basal que apoya la adhesión, la proliferación y la diferenciación. La laminina está compuesta por cadenas A, B1 y B2, que están conectadas por muchos enlaces de disulfuro. Esta laminina se aisló del tumor Engelbreth-Holm-Swarm de ratón.
Precaución
Se recomienda almacenar este producto en alícuotas de trabajo a -20°C.
Nota de preparación
Este producto se suministra a una concentración de 1 − 2 mg/ml en Tris-HCl 50 mM, con NaCl 150 mM. Se filtra utilizando un filtro de 0,2μm. Descongele lentamente esta disolución antes de utilizarla a 2-8°C para evitar la formación de gel. Para utilizar como recubrimiento, dilúyala en una disolución salina equilibrada, recubra la superficie de cultivo con un volumen mínimo e incube a 37 °C durante dos horas. Lave 3 veces con PBS antes de sembrar las células. Los revestimientos de laminina pueden almacenarse durante un mes a 2-8°C.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Artículos
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Protocolos
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
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How long can stocks be frozen at -20C for?
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How will the product L2020 shipped? Will it be shipped in room temperature, on ice, or on dry ice?
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The product L2020 will be shipped using dry ice. Upon receipt, it should be stored at -20 degrees Celsius.
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How is this laminin concentration determined? (by which method?) I tried to measure the concentration of this product by ELISA, and the results always came out half of what is written on the vial.. what is the protein purity?
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Laminin concentration is 1.0 - 2.0 mg protein/ml. The protein content is determined by Lowry method. Please refer the attached document for more details. https://www.sigmaaldrich.com/specification-sheets/131/942/L2020-1MG_________SIGMA____.pdf
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Can I coat Laminin, Product L2020, on top of poly-L-lysine for better adherence?
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When coating coverslips or slides for use with neural stem cells (neurospheres), the following procedure is recommended. Precoat the coverslips with poly-L-lysine (Product No. P5899) at 100 μg/mL. This is incubated for 30 minutes or more at room temperature. The coverslips are then washed with sterile water and allowed to dry. The poly-lysine coated coverslips can be stored at room temperature until ready to use (up to ~1 month). Coverslips are then coated with 30 - 50 μL of warmed laminin, which is at a concentration of 50 μg/mL. Incubate at 37°C for 30 minutes. Remove the laminin, and wash with DME (50 μL) twice. Cells are then added to the coverslips.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What type of laminin is Product L2020?
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In the nomenclature system by Burgeson, this is Laminin 1. The laminins were numbered with Arabic numerals in the order discovered. The previous A, B1, and B2 chains, and their isoforms are listed by the Greek letters, for alpha, beta, and gamma, respectively, followed by an Arabic numeral to identify the isoforms. For example, the first laminin identified from the Engelbreth-Holm-Swarm tumor (EHS) was designated laminin-1 with the chain composition α-1/β-1/γ-1. The genes for these 3 chains are LAMA1, LAMB1, and LAMC1. Reference: Burgeson, R.E., et al., A new nomenclature for the laminins. Matrix Biol., 14, 209-211 (1994).
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How can I store solutions of Laminin, Product L2020?
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The product has a recommended storage temperature of -20°C. Please note, the product needs to be thawed slowly at 2-8°C. If the product has been warmed up at room temperature and it gels, it cannot be reactivated for use. We recommend storage at 2-8°C for up to one week. For longer storage, the product needs to be frozen at -20°C in single-use aliquots.
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What is the purity of Laminin, Product L2020?
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We do not determine a purity of this product. An SDS PAGE gel is run and the pattern (3 bands) is comparable to past lots.
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What is the protein concentration of the solution of Product L2020, Laminin?
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The protein specification for L2020 is 1.0 to 2.0 mg/mL. The exact value can be found on the lot-specific Certificate of Analysis, which can be downloaded from our website.
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