The XNAT2, Extract-N-Amp™ Tissue PCR Kit is not suitable for RNA extraction and is specifically designed for rapid genomic DNA extraction and PCR amplification. Instead, it is recommended to use the GenElute Total RNA purification kit, RNB100.
XNAT2
Extract-N-Amp™ Tissue PCR Kit
sufficient for 100 extractions, sufficient for 100 amplifications
Sinónimos:
Tissue direct PCR kit
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NACRES:
NA.55
UNSPSC Code:
41106303
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Servicio técnico
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Permítanos ayudarleQuality Segment
usage
sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable
color
colorless
input
crude DNA
shipped in
wet ice
storage temp.
−20°C
General description
The Extract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract.
The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the Tissue Preparation Solution and Extraction Solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.
The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the Tissue Preparation Solution and Extraction Solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.
The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by direct PCR (polymerase chain reaction) . A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract.
Application
Biochem/physiol Actions
The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.
Features and Benefits
- Fast – rapid extraction of genomic DNA for PCR in 15 minutes
- Convenient – no long enzymatic digestions or column purification required
- Practical – perfect for quick genomic DNA isolation for genotyping
- Flexible – protocols available for mouse-tails, hair, animal tissue, saliva, and buccal swabs
- Specific – Hot start antibody for highly specific PCR amplification of genomic DNA
- Extract stable at 4 oC for at least 6 months
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Kits contain Tissue Preparation Solution, Extraction Solution, and Extract-N-Amp PCR Reaction Mix.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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Este artículo | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable |
| feature dNTPs included, Direct PCR, hotstart | feature dNTPs included, hotstart, Direct PCR | feature dNTPs included, hotstart, Direct PCR | feature dNTPs included, hotstart, Direct PCR |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| color colorless | color red | color colorless | color colorless |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
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Danger
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Can the kit destroy RNA in the tissue sample? The kit has been used to extract DNA for genotyping, and now the aim is to correlate genotypes with expression levels using qPCR. Is it possible to use the same extract produced by the Extract N Amp Tissue workflow to supply mRNA for qPCR? If not, would any of the kits be able to work in such a manner? The research involves young zebrafish larvae.
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