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T7024

Sigma-Aldrich

N,N,N′,N′-Tetrametiletilendiamina

BioReagent, for molecular biology, ≥99% (GC)

Sinónimos:

1,2- Bis(dimetilamino)-etano, TEMED, TMEDA

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About This Item

Fórmula lineal:
(CH3)2NCH2CH2N(CH3)2
Número de CAS:
110-18-9
Peso molecular:
116.20
Beilstein:
1732991
Número CE:
203-744-6
Número MDL:
MFCD00008335
Código UNSPSC:
41105319
ID de la sustancia en PubChem:
24900242
NACRES:
NA.31
grado:
for molecular biology
Ensayo:
≥99% (GC)
bp:
120-122 °C (lit.)
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

for molecular biology

Nivel de calidad

200

Línea del producto

BioReagent

Ensayo

≥99% (GC)

lim. expl.

9.08 %

índice de refracción

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

densidad

0.775 g/mL at 20 °C (lit.)

actividad extraña

DNase, RNase, protease, none detected

cadena SMILES

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

Clave InChI

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Aplicación

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.[1],[2],[3]
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.[4] TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.[5] Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.[6]
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.[7] TEMED has been used in the HPLC of small organic molecules.[8]

Cantidad

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Palabra de señalización

Danger

Frases de peligro

H225,H302,H314,H331

Clasificaciones de peligro

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

61.7 °F - closed cup

Punto de inflamabilidad (°C)

16.5 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCM7546
BCCL1561
BCCK6320
BCCJ9691
BCCH5634

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A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
Arvind Chandrasekaran et al.
Lab on a chip, 19(17), 2786-2798 (2019-07-25)
To study respiratory diseases, in vitro airway epithelial models are commonly implemented by culturing airway cells on a porous surface at an air-liquid interface (ALI). However, these surfaces are often supraphysiologically stiff, which is known to affect the organization, maturation
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Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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