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T6763

Trypsin Acetylated from bovine pancreas

Name: Trypsin Acetylated from bovine pancreas
Brand: SIGMA

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

Sinónimos:

Acetyltrypsin

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UNSPSC Code:

12352204

NACRES:

NA.54

Número CE:

3.4.21.4 (BRENDA, IUBMB)

MDL number:

MFCD00082094

Specific activity:

≥8,500 BAEE units/mg protein (biuret)

Biological source:

bovine pancreas

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Propiedades

biological source

bovine pancreas

Quality Segment

200

type

Type V-S

form

powder

specific activity

≥8,500 BAEE units/mg protein (biuret)

UniProt accession no.

Q29463

storage temp.

−20°C

Gene Information

cow ... TRYP8(282603)

Descripción

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. [1] Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter. [2]

Biochem/physiol Actions

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Preparation Note

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Other Notes

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

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Este artículo	T8802	T1005	T1143
Sigma-Aldrich T6763 Trypsin Acetylated from bovine pancreas	Sigma-Aldrich T8802 Tripsina from bovine pancreas	Sigma-Aldrich T1005 Tripsina from bovine pancreas	Sigma-Aldrich T1143 Trypsinogen from bovine pancreas
Gene Information cow ... TRYP8(282603)	Gene Information -	Gene Information -	Gene Information bovine ... TRYP8(282603)
specific activity ≥8,500 BAEE units/mg protein (biuret)	specific activity ≥10,000 BAEE units/mg protein	specific activity ≥6,000 BAEE units/mg protein	specific activity ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)
biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas
form powder	form essentially salt-free, lyophilized powder	form lyophilized powder	form essentially salt-free, lyophilized powder
UniProt accession no. Q29463	UniProt accession no. -	UniProt accession no. -	UniProt accession no. Q29463
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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