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T6424

Sigma-Aldrich

Trypsin from human pancreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

Sinónimos:

Serine Protease 1

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About This Item

Número de CAS:
9002-07-7
Comisión internacional de enzimas:
3.4.21.4 (BRENDA, IUBMB)
Número CE:
232-650-8
Número MDL:
MFCD01323069
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

human pancreas

Nivel de calidad

200

Formulario

salt-free, lyophilized powder

envase

vial of ≥1,000 BAEE units

Nº de acceso UniProt

P07477
P07478
P35030

temp. de almacenamiento

−20°C

Información sobre el gen

human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)

Descripción general

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Aplicación

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Definición de unidad

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Cantidad

Minimum 1,000 BAEE units/vial

Nota de análisis

Protein determined by E1%/280.

Otras notas

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Inhibidor

Referencia del producto
Descripción
Precios

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Benzamidine hydrochloride hydrate, ≥99%

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Trypsin inhibitor from Glycine max (soybean), lyophilized powder

M6159

α2-Macroglobulin from human plasma, BioUltra, ≥98% (SDS-PAGE)

D7910

3,4-Dichloroisocoumarin, serine protease inhibitor

D0879

Diisopropylfluorophosphate

L2023

Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial

A6191

Antipain dihydrochloride from microbial source, protease inhibitor

T9777

Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder

A1153

Aprotinina from bovine lung, lyophilized powder, 3-8 TIU/mg solid

P7626

Fluoruro de fenilmetansulfonilo, ≥98.5% (GC)

A9024

α1-Antitrypsin from human plasma, salt-free, lyophilized powder

A6150

α1-Antitrypsin from human plasma, salt-free, lyophilized powder

T2011

Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)

sustrato

Referencia del producto
Descripción
Precios

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H334,H335

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000426761
0000426761
0000376372
0000330530
0000330530

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Visite la Librería de documentos

Jia Liu et al.
Nature protocols, 16(7), 3522-3546 (2021-06-06)
Cellular heterogeneity is pervasive and of paramount importance in biology. Single-cell analysis techniques are indispensable for understanding the heterogeneity and functions of cells. Low-copy-number proteins (fewer than 1,000 molecules per cell) perform multiple crucial functions such as gene expression, cellular
Kelly A Dryden et al.
Journal of molecular biology, 422(5), 650-658 (2012-06-30)
Human astroviruses (HAstVs) are a major cause of gastroenteritis. HAstV assembles from the structural protein VP90 and undergoes a cascade of proteolytic cleavages. Cleavage to VP70 is required for release of immature particles from cells, and subsequent cleavage by trypsin
Industrial Enzymes and Their Applications (1998)
Cynthia Luz Marcelo et al.
BMC dermatology, 12, 8-8 (2012-06-26)
Primary keratinocytes derived from epidermis, oral mucosa, and urothelium are used in construction of cell based wound healing devices and in regenerative medicine. This study presents in vitro technology that rapidly expands keratinocytes in culture by growing monolayers under large
Mian Zhou et al.
Nature, 495(7439), 111-115 (2013-02-19)
Codon-usage bias has been observed in almost all genomes and is thought to result from selection for efficient and accurate translation of highly expressed genes. Codon usage is also implicated in the control of transcription, splicing and RNA structure. Many
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Protocolos

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

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