T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

• Número de CAS: 52500-60-4
• Número MDL: MFCD00132423
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: Escherichia coli
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: essentially salt-free, lyophilized powder
• actividad específica: ≥3 units/mg protein
• mol peso: 11.7 kDa
• técnicas: activity assay: suitable
• solubilidad: water: 1.90-2.10 mg/mL, clear, colorless
• Nº de acceso Protein ID: M26133
• Nº de acceso UniProt: P0AA25
• aplicaciones: cell analysis
• temp. de almacenamiento: −20°C
• Información sobre el gen: Escherichia coli K12 ... trxA(948289)

Descripción

Descripción general

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Aplicación

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Acciones bioquímicas o fisiológicas

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

Otras notas

GenBank^® Accession Number M26133.

Definición de unidad

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Información legal

GenBank is a registered trademark of United States Department of Health and Human Services

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)