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RAB0362

Human MMP-1 ELISA Kit

Name: Human MMP-1 ELISA Kit
Brand: SIGMA

for cell and tissue lysates

Sinónimos:

MMP-1, Matrix metalloproteinase-1

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NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... MMP1(4312)

Input:

sample type tissue lysate
sample type cell lysate

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

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Propiedades

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP1(4312)

Descripción

General description

The Human MMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-1 cell lysate and tissue lysate.

Immunogen

Recombinant Human MMP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular diseases, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with rheumatoid arthritis and osteoarthritis. MMP-1 is linked with juvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease. MMP-1 might facilitate fibrous plaque disruption via degradation of interstitial collagens and thinning of the fibrous cap.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Este artículo	RAB0361	RAB0368	RAB0370
Sigma-Aldrich RAB0362 Human MMP-1 ELISA Kit	Sigma-Aldrich RAB0361 Human MMP-1 ELISA Kit	Sigma-Aldrich RAB0368 Mouse MMP-3 ELISA Kit	Sigma-Aldrich RAB0370 Human MMP-8 ELISA Kit
description for cell and tissue lysates	description for serum, plasma, cell culture supernatant and urine	description for serum, plasma and cell culture supernatant	description for serum, plasma, conditioned medium, urine
species reactivity human	species reactivity human	species reactivity mouse	species reactivity human
Gene Information human ... MMP1(4312)	Gene Information human ... MMP1(4312)	Gene Information mouse ... Mmp3(17392)	Gene Information human ... MMP8(4317)
input sample type tissue lysate sample type cell lysate	input sample type plasma sample type cell culture supernatant(s) sample type serum sample type urine	input sample type serum sample type cell culture supernatant(s) sample type plasma	input sample type plasma sample type serum sample type urine sample type conditioned medium
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 24.69-18000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 24.69-18000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 13.72-10000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 6 pg/mL standard curve range: 8.23-6000 pg/mL
detection method colorimetric	detection method colorimetric	detection method colorimetric	detection method colorimetric
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable

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Los componentes del kit también están disponibles por separado

Referencia del producto

Descripción

SDS & Precios

RABDIL6
ELISA 5X Sample Diluent Buffer (Item D2)
SDS

RABELADE
ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
SDS

RABLYSIS1
2X Cell Lysis Buffer (Item J)
SDS

RABSTOP3
ELISA Stop Solution (Item I)
SDS

RABTMB3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
SDS

RABWASH4
20X Wash Buffer (Item B)
SDS

Pictogramas

GHS05

Palabra de advertencia

Warning

Códigos de peligro

H290

Indicaciones de precaución

P234 - P390

Hazard Classifications

Met. Corr. 1

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

Clase de almacenamiento

12 - Non Combustible Liquids

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Certificados de análisis (COA)

Lot/Batch Number

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