RAB0362

Human MMP-1 ELISA Kit

for cell and tissue lysates

• Sinónimos: MMP-1, Matrix metalloproteinase-1
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... MMP1(4312)
• Input: sample type tissue lysate; sample type cell lysate
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Propiedades

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type tissue lysate; sample type cell lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 8 pg/mL; standard curve range: 24.69-18000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MMP1(4312)

Descripción

General description

The Human MMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-1 cell lysate and tissue lysate.

Immunogen

Recombinant Human MMP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular diseases, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with rheumatoid arthritis and osteoarthritis. MMP-1 is linked with juvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease. MMP-1 might facilitate fibrous plaque disruption via degradation of interstitial collagens and thinning of the fibrous cap.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

• RABDIL6

  ELISA 5X Sample Diluent Buffer (Item D2)

  SDS
• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDS
• RABLYSIS1

  2X Cell Lysis Buffer (Item J)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Información de seguridad

• Pictogramas: GHS05
• Palabra de advertencia: Warning
• Códigos de peligro: H290
• Indicaciones de precaución: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• Clase de almacenamiento: 12 - Non Combustible Liquids