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M3769

Medium 199

Name: Medium 199
Brand: SIGMA

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Sinónimos:

M199 Medium, TCM 199

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Acerca de este artículo

NACRES:

NA.71

UNSPSC Code:

12352207

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Propiedades

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Preparation Note

Formulated to contain 9.4 grams of powder per liter of medium.

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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Este artículo	M2520	M2154	M0393
Sigma-Aldrich M3769 Medium 199	Sigma-Aldrich M2520 Medium 199	Sigma-Aldrich M2154 Medium 199	Sigma-Aldrich M0393 Medium 199
form powder	form powder	form liquid	form powder
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components L-glutamine: no phenol red: no HEPES: no sodium pyruvate: no Earle’s salts (5% CO₂): yes NaHCO₃: no	components L-glutamine: yes phenol red: yes Earle’s salts (5% CO₂): yes NaHCO₃: no sodium pyruvate: no HEPES: 25 mM	components sodium pyruvate: no L-glutamine: no NaHCO₃: yes HEPES: no phenol red: yes Earle’s salts (5% CO₂): yes	components HEPES: no Hanks’ salts (2% CO₂): yes sodium pyruvate: no NaHCO₃: no L-glutamine: yes phenol red: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 500	Quality Level 400	Quality Level 500

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Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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