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M2279

Medio esencial mínimo Eagle

Name: Medio esencial mínimo Eagle
Brand: SIGMA

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Minimum Essential Medium Eagle

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Sinónimos:

MEM

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UNSPSC Code:

12352207

NACRES:

NA.75

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Propiedades

Nombre del producto

Medio esencial mínimo Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO₃: yes
Earle’s salts (5% CO₂): yes

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used to culture the:

explants of aortic valve leaflets
human liver cancer cell line (HepG2) cells[1]
human fetal brain progenitor-derived astrocyte cells (PDA)
breast cancer cell line (MCF-7)
human glioblastoma cell line (T98G)[2]

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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Este artículo	M5650	M0200	M8042
Sigma-Aldrich M2279 Medio esencial mínimo Eagle	Sigma-Aldrich M5650 Medio esencial mínimo Eagle	Sigma-Aldrich M0200 Medio esencial mínimo Eagle	Sigma-Aldrich M8042 Medio esencial mínimo Eagle
form liquid	form liquid	form liquid	form liquid
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes	components phenol red: yes NaHCO₃: yes L-glutamine: no sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes	components phenol red: 0.011 g/L glucose: 1.0 g/L L-glutamine: 0.292 g/L NaHCO₃: 2.2 g/L	components Hanks’ salts (2% CO₂): no glucose: yes sodium pyruvate: yes phenol red: yes HEPES: no L-glutamine: no stable glutamine: no Earle’s salts (5% CO₂): yes NaHCO₃: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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