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Merck
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L8146

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

peroxidase conjugate, lyophilized powder

Sinónimos:

Ulex europaeus agglutinin, UEA-I

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

Ulex europaeus

Nivel de calidad

200

conjugado

peroxidase conjugate

Ensayo

70.00-90.00% (Lowry)

Formulario

lyophilized powder

potencia

<40 μg/mL agglutination activity

peroxidase activity

≥15 units/mg protein

composición

Protein, ~80% Lowry

técnicas

western blot: suitable

temp. de almacenamiento

2-8°C

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Aplicación

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Acciones bioquímicas o fisiológicas

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Envase

Package size based on protein content.

Ligadura / enlace

Conjugates are prepared from affinity purified lectin.

Definición de unidad

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Forma física

Contains sodium citrate buffer salts

Nota de preparación

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

Nota de análisis

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000395425
0000395425
098M4057V
098M4057V
11180425

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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
S Avrameas et al.
Immunochemistry, 8(12), 1175-1179 (1971-12-01)
Nadine Mikuda et al.
The Journal of pathology, 251(2), 160-174 (2020-03-30)
The IκB kinase (IKK)-NF-κB signaling pathway plays a multifaceted role in inflammatory bowel disease (IBD): on the one hand, it protects from apoptosis; on the other, it activates transcription of numerous inflammatory cytokines and chemokines. Although several murine models of
F Bucardo et al.
Zoonoses and public health, 63(8), 600-607 (2016-05-14)
Information about porcine norovirus (PoNoV), genetically similar to human NoV (HuNoV), is limited from rural areas where household-raised pigs are heavily exposed to faecal material which could facilitate transmission. Histo-blood group antigens (HBGAs) are known susceptibility factors to NoV in
K Nozaki et al.
Journal of neurology, neurosurgery, and psychiatry, 80(8), 904-908 (2009-02-26)
To study the clinical and pathological correlations of neuromuscular patients with a high aldolase and normal creatine kinase (CK) in serum at presentation or during a symptomatic exacerbation. Records and muscle biopsies were retrospectively reviewed in a consecutive series of
Xiaohu Fan et al.
Human gene therapy, 21(7), 877-890 (2010-02-19)
The ABO histo-blood group system is the most important antigen system in transplantation medicine, yet no small animal model of the ABO system exists. To determine the feasibility of developing a murine model, we previously subcloned the human alpha-1,2-fucosyltransferase (H-transferase
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