The long term stability of this product in solution has not been investigated. Stock solutions prepared at a minimum of 1 mg/mL in water or PBS may be stored in aliquots up to 3 months at -20 °C when protected from light. Avoid repeated freeze/that cycles.
L4895
Lectin from Triticum vulgaris (wheat)
FITC conjugate, lyophilized powder
Sinónimos:
WGA, Wheat germ agglutinin
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Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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Servicio técnico
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FITC conjugate
Quality Segment
form
lyophilized powder
potency
<50 μg/mL agglutination activity
composition
Protein, ~50% Lowry
extent of labeling
~2 mol FITC per mol protein
storage temp.
2-8°C
General description
Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.
Application
Lectin from Triticum vulgaris (wheat) has been used:
- to treat transfected cells for immunofluorescence
- to stereotaxically inject into mice for neuronal tracing
- in immunohistochemistry for the analysis of myocytes
For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Biochem/physiol Actions
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains phosphate buffer salts
Preparation Note
Conjugates are prepared from affinity purified lectin.
1 of 1
Este artículo | |||
|---|---|---|---|
| form lyophilized powder | form saline suspension | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| potency <50 μg/mL agglutination activity | potency - | potency - | potency - |
| conjugate FITC conjugate | conjugate agarose conjugate | conjugate biotin conjugate | conjugate - |
| composition Protein, ~50% Lowry | composition - | composition Protein, ≥80% E1%/280 | composition - |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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After reconstitution, how should lectin be stored and for how long at given temperature?
1 answer-
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Please is this product use in staining Arbuscular mycorrhizal fungi in root, for confocal microscopy examination?
1 answer-
This product has not been tested specifically for use in fungal staining in plant tissue. However, FITC-labeled wheat germ agglutinin or lectin has been cited in the literature for such applications. Please see the link below to review one such publication:
https://iris.unito.it/bitstream/2318/1740844/1/Carotenuto%20et%20al%202.pdfHelpful?
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Does Sigma provide a stain to visualize cell borders for human Cardiomyocytes derived from the GM23338 iPSC cell line?
1 answer-
There is no recommended stain for cells from this type of cell line. However, for visualizing typical cardiomyocyte cell borders, one may consider L4895, as referenced by Vanhoutte D et al, Nat Commun. 2021 Jun 24;12(1):3928. L4895 is Sigma-Aldrich Lectin from Triticum vulgaris (wheat). The article titled "Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy" in Nat Commun. 2021 Jun 24;12(1):3928 may serve as a point of reference.
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