L7391

Anti-LIS1 antibody, Mouse monoclonal

clone LIS1-338, purified from hybridoma cell culture

• Número MDL: MFCD03457563
• Código UNSPSC: 12352203
• ID de la sustancia en PubChem: 24896475
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: LIS1-338, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 46 kDa
• reactividad de especies: zebrafish, chicken, rat, human, bovine, mouse
• concentración: ~2 mg/mL
• técnicas: immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using a rat brain cytosol preparation
• isotipo: IgG1
• Nº de acceso UniProt: P43034
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• cadena SMILES: CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O
• InChI: 1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+
• Clave InChI: ZQTAMPRZFOOEEP-AVQMFFATSA-N
• Información sobre el gen: human ... PAFAH1B1(5048) ; mouse ... Pafah1b1(18472) ; rat ... Pafah1b1(83572)

Descripción

Descripción general

Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.

The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.

Especificidad

By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).

Inmunógeno

recombinant human LIS1 protein.

Aplicación

Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.

Acciones bioquímicas o fisiológicas

LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.

The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable