If not used immediately, parafilm wrap the ECM coated culture plates and store at 2-8°C until ready to
use. Use the ECM coated culture plates within 3-4 days.
E1270
Gel ECM from Engelbreth-Holm-Swarm murine sarcoma
liquid, BioReagent, suitable for cell culture
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
Sinónimos:
Matriz EHS
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Servicio técnico
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Permítanos ayudarleproduct line
BioReagent
Quality Level
form
liquid
concentration
8-12 mg/mL
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
Gene Information
Application
El gel ECM puede utilizarse con células epiteliales, células endoteliales, células musculares, células nerviosas y células tumorales.
Biochem/physiol Actions
El gel ECM experimentará polimerización termoactivada cuando se lleve a una temperatura de 20-40°C, formando una membrana basal reconstituida. Las células PC12 mostrarán formación axónica en dos días si se cultivan en una capa fina de este gel ECM.
Cada colonia de ratones utilizada para la producción de este producto se examina sistemáticamente para detectar varios patógenos. Probado y encontrado negativo para LDEV.
Cada colonia de ratones utilizada para la producción de este producto se examina sistemáticamente para detectar varios patógenos. Probado y encontrado negativo para LDEV.
Preparation Note
Este producto se prepara a partir del sarcoma Engelbreth-Holm-Swarm en ratones; dializado contra cloroformo. El gel debe descongelarse durante la noche a 2-8 °C antes de utilizarlo y debe dispensarse a una placa multipared utilizando una placa y pipetas que hayan sido previamente enfriadas a 2-8 °C. El gel también puede diluirse hasta el doble con medio de Eagle modificado de Dulbecco, y debe hacerse antes de añadir el gel a la placa. El producto se gelificará en 5 minutos a 20°C. Para manipulaciones prolongadas, el trabajo debe realizarse por debajo de 10 °C. Las células pueden sembrarse en la parte superior de una fina capa de gel de 0,5 mm o cultivarse en el interior de una capa de 1 mm. Si cultiva las células en el interior, agregue las células al gel antes de colocar en placa a una densidad recomendada de 3-4 x 104 células por ml. Para disociar las células de este gel, utilice proteasa en PBS sin calcio, magnesio o EDTA, a una concentración de 0,6-2,4 unidades por ml.
Other Notes
El ECM se preparó a una concentración proteica de 8-12 mg/ml (en DMEM), que contenía laminina como componente principal, colágeno de tipo IV, proteoglucano sulfato de heparina, entactina y otros componentes menores. La adición de colágeno de tipo IV al gel de ECM aumenta la polimerización.
El producto contiene 50 mg/l de sulfato de gentamicina.
El producto contiene 50 mg/l de sulfato de gentamicina.
Disclaimer
Para su conservación a largo plazo, mantenga el producto a -20°C. El gel ECM puede conservarse a una temperatura de 2-8°C durante un máximo de 72 horas.
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form lyophilized powder | form liquid |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in ambient |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| product line BioReagent | product line BioReagent | product line BioReagent | product line - |
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| E1270-1ML | 04061833601600 |
| E1270-5X10ML | 04061833601617 |
| E1270-10ML | 04061833601594 |
| E1270-5ML | 04061838447104 |
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Can ECM Gel from Engelbreth-Holm-Swarm murine sarcoma be coated on a plate and stored overnight at 4C in parafilm to be used the next day?
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Is this product suitable for implantation of tumors in mice or does the phenol red preclude it?
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Product E1270 Sigma-Aldrich ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been validated for cell culture applications only. This product is designed for coating tissue culture glassware. It has not been validated for in vivo use.
Please refer to the following link below for more information:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/3d-cell-culture/ecm-gel-product-protocolsHelpful?
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Can this be used for xenograft studies in immuncompromised mice.
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This product is not tested on xenograft studies in immunocompromised mice. It is not recommended to be used for that application.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?
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Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.
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The data sheet for Product E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, says that it may be diluted up to twofold. What does that mean?
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ECM gel can be diluted twofold with cold Dulbecco's Modified Eagle's Medium (DME). This means 1 part ECM gel and 1 part DME.
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Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?
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Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.
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What is the calcium concentration in Products E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?
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The approximate concentration of CaCl2 in the ECM solution is 0.265 mg/mL.
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Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?
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Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product. Product No. E6909, ECM gel, growth factor reduced, is phenol red free.
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How can I retrieve my cells from Product E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?
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Dispase (Product No. D4818) is a Bacillus-derived neutral protease that is recommended for recovering cells cultured on ECM (Product No. E1270) or Matrigel. Dispase will yield a single cell suspension far more effectively than trypsin, collagenase, or other proteolytic enzymes. Dispase is gentle and will not damage cells harvested for sub-cultivation or other study.
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