GE17-6002-33

HiTrap^® IEX Selection Kit

Cytiva 17-6002-33

• Sinónimos: IEX Selection Kit
• Código UNSPSC: 23151816
• NACRES: NA.56

Propiedades

• ligand: quaternary amine
• descripción: Ion Exchanger Type (value)
• envase: pkg of 7 ea
• fabricante / nombre comercial: Cytiva 17-6002-33
• Parámetros: 42 psi
• lecho tamaño: 7 mm × 25 mm
• lecho volumen: 1 mL
• columna D.I.: 7 mm
• Matriz: 6% cross-linked agarose
• tamaño de partícula: 45-165 μm
• diámetro promedio: 90 μm
• cleaning: 2-14
• Intervalo de trabajo: 2-12
• idoneidad: suitable for bioprocess medium

Descripción

Descripción general

HiTrap^® IEX Selection Kit consists of seven HiTrap^® columns, prepacked with different Sepharose^™ Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.

Q, SP, DEAE, and CM Sepharose^™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose^™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose^™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose^™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose^™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.

Características y beneficios

• Fast and easy selection with seven different ion-exchange ligands on Sepharose^™ Fast Flow and Sepharose^™ XL, prepacked in 1 mL HiTrap^® columns.
• Offers a fast, easy and convenient way screen for the optimal media for a given application .
• Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
• Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids