L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
G2150
L-Glutamina solution
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
Sinónimos:
L-Glutamine for cell culture
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352209
eCl@ss:
32160406
ID de la sustancia en PubChem:
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
grado
Hybri-Max™
Nivel de calidad
esterilidad
sterile-filtered
Ensayo
≥99% (TLC)
Formulario
solution
técnicas
cell culture | hybridoma: suitable
impurezas
endotoxin, tested
color
clear colorless
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
cadena SMILES
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
Clave InChI
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Información sobre el gen
rat ... Ggt1(116568)
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Categorías relacionadas
Descripción general
La L-glutamina es un aminoácido esencial que es un componente crucial de los medios de cultivo donde actúa como una fuente importante de energía para las células en cultivo. La L-glutamina es muy estable en forma de polvo seco y de disolución congelada. En los medios líquidos o las disoluciones de reserva, sin embargo, la L-glutamina se degrada con relativa rapidez. El rendimiento celular óptimo normalmente requiere el enriquecimiento de los medios con L-glutamina antes de su uso.
Aplicación
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Nota de preparación
Prepared in cell culture grade water.
Información legal
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Aplicación
Referencia del producto
Descripción
Precios
Producto relacionado
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Importance and uses of glutamine in hybridoma and mammalian cell culture
Importance and uses of glutamine in hybridoma and mammalian cell culture
Importance and uses of glutamine in hybridoma and mammalian cell culture
Importance and uses of glutamine in hybridoma and mammalian cell culture
Chromatograms
application for HPLC-
Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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