F6636

Nutrient Mixture F-12 Ham

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

• Sinónimos: Ham’s F-12
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217417

Propiedades

• form: powder
• technique(s): cell culture | hybridoma: suitable, cell culture | mammalian: suitable
• components: phenol red: 0.00125 g/L; L-glutamine: 0.292 g/L; sodium pyruvate: 0.22 g/L; glucose: 1.802 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

Application

Coon′s modification of Ham′s F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Preparation Note

Formulated to contain 11.5 grams of powder per liter of medium.

Supplement with 2.68 g/L sodium bicarbonate.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)