Various solvents, including ethanol, are reported to inhibit forskolin-mediated activation of adenylate cyclase. Dimethyl sulfoxide (DMSO) is the recommended solvent; at a final concentration of 5% or less, little if any inhibition of adenylate cyclase activation will occur. A 50 mM stock solution of forskolin can be prepared by solubilizing 10 mg of forskolin in 488 µL of fresh, high purity, and anhydrous DMSO. After reconstitution, aliquot and freeze at -20°C. The 50 mM stock solution can be diluted 1:100 in DMSO to achieve a 500 µM working stock solution.
F3917
Forskolina
For use in molecular biology applications
Sinónimos:
7β-Acetoxi-8,13-epoxi-1α,6β,9α-trihidroxilabd-14-en-11-ona, Coleonol, Colforsin
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About This Item
Fórmula empírica (notación de Hill):
C22H34O7
Número de CAS:
Peso molecular:
410.50
Beilstein:
1692716
Número CE:
Número MDL:
Código UNSPSC:
12352200
ID de la sustancia en PubChem:
NACRES:
NA.52
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Productos recomendados
origen biológico
Coleus forskohlii
descripción
for activation of T cells
Línea del producto
BioReagent
Ensayo
≥98% (HPLC)
Formulario
powder
mol peso
410.50 g/mol
color
white to off-white
solubilidad
ethanol: soluble
idoneidad
suitable for inhibits interleukin 2 (IL-2) production in Jurkat cells
temp. de almacenamiento
room temp
cadena SMILES
CC(=O)O[C@H]1[C@@H](O)[C@H]2C(C)(C)CC[C@H](O)[C@]2(C)[C@@]3(O)C(=O)C[C@@](C)(O[C@]13C)C=C
InChI
1S/C22H34O7/c1-8-19(5)11-14(25)22(27)20(6)13(24)9-10-18(3,4)16(20)15(26)17(28-12(2)23)21(22,7)29-19/h8,13,15-17,24,26-27H,1,9-11H2,2-7H3/t13-,15-,16-,17-,19-,20-,21+,22-/m0/s1
Clave InChI
OHCQJHSOBUTRHG-KGGHGJDLSA-N
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Descripción general
La forskolina es un diterpenoide permeable a la célula aislada de Coleus forskohlii.
Acciones bioquímicas o fisiológicas
Diterpenoide permeable a la célula que posee propiedades antihipertensivas, inotropas positivas y de activación de la adenilil ciclasa. Muchos de sus efectos biológicos se deben a su activación de la adenilil ciclasa y el aumento resultante de la concentración de AMPc intracelular. La forskolina afecta a las corrientes de calcio e inhibe la MAP cinasa.
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Dermal
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Gloves
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Protocolos
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
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How can i prepare 500uM Forskolin (10mg) and where can the prepared solution be stored?
1 answer-
Helpful?
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What is the shelf life of the solid form of forskolin?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
Helpful?
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How should I store a DMSO or ethanol solution of Product F3917, Forskolin?
1 answer-
Forskolin solutions at 5 mg/mL in either ethanol or DMSO have been found to be stable for at least 6 months at room temperature, as determined by HPLC analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I make an aqueous solution of Product F3917, Forskolin?
1 answer-
Forskolin is soluble in water (with 2% ethanol) up to 0.2 mM by first dissolving in ethanol at 5 mg/mL and performing subsequent dilutions in water. Similar results were obtained with DMSO.
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Will residual solvents inhibit forskolin activation of adenylate cyclase?
1 answer-
Various solvents, including ethanol, inhibit the forskolin activation of adenylate cyclase. DMSO is recommended for preparing forskolin solutions, because at concentrations of 5% or less of DMSO, there is little if any inhibition of forskolin activation.
Helpful?
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