For adjustment to approximately pH 8, add equilibration buffer at a ratio of 6.5 mL per 100 mL of phenol. Use 650 µL of buffer for 10 mL of phenol or 65 µL of buffer for 1 mL of phenol. The expected adjusted pH is 7.9 ± 0.2.
This phenol solution (product P4557) includes a separate bottle of equilibration buffer. After buffer addition, allow approximately 6 hours at 2–8 °C for the bottom phenol phase to become clear. Mixing by inversion can introduce air bubbles and cause temporary haziness. A hazy interface between the phenol and aqueous phases can remain after separation.
P4557
Fenol solution
BioReagent, Molecular Biology
Sinónimos:
Tris-equilabrated Phenol
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Acerca de este artículo
Fórmula lineal:
C6H5OH
Número CAS:
Peso molecular:
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-632-7
Beilstein/REAXYS Number:
6428463
MDL number:
Assay:
≥99.0% (GC)
Grade:
Molecular Biology
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Molecular Biology
Quality Segment
product line
BioReagent
assay
≥99.0% (GC)
form
liquid
dilution
(for analytical testing)
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
SMILES string
Oc1ccccc1
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
Gene Information
human ... GABRA1(2554)
General description
Una disolución de fenol equilibrada con Tris es un líquido transparente cubierto con una capa de tampón Tris. Las disoluciones de fenol se pueden utilizar para una amplia variedad de aplicaciones de biología molecular. Los compuestos orgánicos como los fenoles tienen un anillo carbocíclico aromático con un grupo hidroxilo unido directamente a él. El nombre común del monohidroxibenceno es fenol.
Application
La disolución de fenol se ha utilizado:
- como componente de la mezcla de reacción de digestión para preparar plásmidos sin ARNasas destinados a la traducción in vitro en un lisado de reticulocitos de conejo
- para la extracción de ADN de hojas de roseta Arabidopsis thaliana
- en la extracción de ADN de sangre en papel conservante por protocolo estándar de fenol-cloroformo
- como componente del tampón de extracción para extraer ARN de la pulpa de la fruta
- para la extracción de proteínas de las raíces
- para el aislamiento del ADN fecal
- para la extracción de ARN enjaulado
Biochem/physiol Actions
El método de extracción fenol-cloroformo se emplea ampliamente para extraer ácidos nucleicos. El fenol no inhibe por completo la actividad ARNasa, pero su característica única lo hace beneficioso para el aislamiento de ácidos nucleicos cuando se usa en combinación con cloroformo y alcohol.
Other Notes
- 1 botella de fenol equilibrado con Tris (referencia P4557)
- Tampón de equilibrio (referencia B5658)
1 of 1
Este artículo | |||
|---|---|---|---|
| description BioReagent, Molecular Biology | description certified reference material, 500 μg/mL in methanol | description 5000 μg/mL in methanol, certified reference material | description Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology |
| grade Molecular Biology | grade TraceCERT®, certified reference material | grade TraceCERT®, certified reference material | grade Molecular Biology |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| assay ≥99.0% (GC) | assay - | assay - | assay - |
| form liquid | form - | form - | form liquid |
| pH 7.7-8.1 (25 °C) | pH - | pH - | pH 4.1-4.5 (25 °C) |
| dilution (for analytical testing) | dilution - | dilution - | dilution - |
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Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Órganos diana
Nervous system,Kidney,Liver,Skin
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 2
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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The instructions say that if I add the full volume of buffer (B5658, 6.5ml), the pH will be around 10. How much should I add if I want to adjust it to pH 8?
1 answer-
Helpful?
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What happens if I mix the two layers of phenol before pipetting? I prepared FIAC this way
1 answer-
Mixing the two phenol layers does not damage this product. Phase separation can occur during handling. If layers mix, allow the solution to stand until a clear phase separation is re-established before pipetting. Temporary haziness or a visible interface after mixing is normal. Pipetting should occur only after full separation to avoid aqueous carryover and ensure consistent performance. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/087/p4557dat.pdfHelpful?
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How long can you store phenol for, after adding the buffer?
1 answer-
Following the addition of buffer, the solution may be stored for 6 months at 2–8 °C. For extended storage after opening, with or without the addition of buffer, the material may be stored at –20 °C, protected from light. This information is available in the product datasheet. Please see the link below to review additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/087/p4557dat.pdfHelpful?
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Esta botella de Fenol (10 mM TrisHCl pH 8.0) viene junto con una botella pequeña de tampón Tris a pH 10.5 for saturated phenol. Se supone que debo añadírsela a la botella de fenol para ajustar a 10 mM TrisHCl?
1 answer-
Yes, this phenol solution includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. The entire contents of the bottle of the buffer should be added to the phenol.
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What is the export packaging? Is it in thermocol/Foam packaging? Can you share the packaging Image.
1 answer-
Both package sizes are bottles that are sealed in a polyamide bag. This bag is placed, with additional absorbent material and padding, in a metal can with a pull tap. The final packaged item is placed in the shipping box that is most compatible with the order. This product is shipped at ambient temperature.
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What is the molarity of Phenol solution, Product P4557?
1 answer-
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the final pH of Phenol solution, Product P4557, after adding the buffer?
1 answer-
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
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What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
1 answer-
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
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I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
1 answer-
The top phase is Tris buffer, so use the lower/bottom phase.
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